"Shin Tennis no Ōji-sama OVA vs. Genius 10" continues the narrative of the "Prince of Tennis" series, focusing on the U-17 World Cup arc. The story centers on Ryoma Echizen and his fellow Japanese middle school tennis players as they face off against the Genius 10, an elite group of high school players representing Japan's U-17 training camp. The Genius 10, led by Byodoin Houou, are the top-ranked players in the camp, and their dominance is challenged by the middle schoolers, who aim to prove their worth and secure spots on the national team.

The OVA is structured around a series of intense matches between the middle schoolers and the Genius 10. Each match highlights the unique skills and strategies of the players, with the middle schoolers pushing their limits to overcome the overwhelming talent of their high school opponents. Key characters such as Ryoma, Tezuka Kunimitsu, and Yukimura Seiichi showcase their growth and determination, while members of the Genius 10, including Byodoin, Tokugawa Kazuya, and Oni Juujiro, demonstrate their superior abilities and experience.

The narrative emphasizes themes of rivalry, perseverance, and the pursuit of excellence in tennis. The matches are not only physical battles but also psychological contests, with players employing advanced techniques and mental fortitude to outwit their opponents. The OVA builds on the established dynamics of the series, deepening character relationships and exploring the competitive spirit that drives each player. The stakes are high, as the outcomes of these matches determine who will represent Japan in the upcoming U-17 World Cup.

Throughout the OVA, the middle schoolers face significant challenges, often being outmatched in terms of raw power and experience. However, their creativity, teamwork, and relentless drive allow them to compete at the highest level. The Genius 10, while formidable, are not invincible, and their vulnerabilities are exposed as the middle schoolers adapt and evolve during the matches. The story culminates in a climactic showdown that sets the stage for the next phase of the U-17 World Cup arc, leaving viewers eager to see how the players will fare on the international stage.