"Koihime Musou" is an anime adaptation of the visual novel "Koihime†Musō," which reimagines the historical Chinese epic "Romance of the Three Kingdoms" with a gender-swapped cast and a comedic, romantic twist. The story is set in a fictionalized version of ancient China, where the land is plagued by corruption and chaos. The protagonist, Kan'u Unchou, is a young woman with a strong sense of justice who embarks on a journey to restore peace. Along the way, she encounters other female warriors, each inspired by historical figures from the Three Kingdoms era, who join her cause.

The narrative begins with Kan'u saving a village from bandits, where she meets Chouhi Yokutoku, a spirited and impulsive fighter, and Bachou Mouki, a calm and composed strategist. Together, they form a bond and decide to fight against the oppressive forces destabilizing the land. As they travel, they recruit additional allies, including the noble and determined Ryuubi Gentoku, who seeks to unite the people under a just rule. The group faces various challenges, including rival factions and internal conflicts, as they strive to bring order to the war-torn nation.

The series blends action, comedy, and light romantic elements, with a focus on the relationships between the characters. Each character is loosely based on a figure from "Romance of the Three Kingdoms," such as Kan'u representing Guan Yu, Chouhi as Zhang Fei, and Ryuubi as Liu Bei. The anime explores their personal motivations and growth while maintaining a lighthearted tone, often incorporating humorous situations and exaggerated character traits. Despite its comedic approach, the story occasionally touches on themes of loyalty, friendship, and the struggle for justice in a turbulent world. The series concludes with the group continuing their journey, leaving their ultimate success open-ended.