"Star Ship Operators" is a science fiction anime series set in a future where humanity has colonized multiple star systems. The narrative centers on the crew of the Amaterasu, a training starship operated by the Kingdom of Kibi's Royal Space Force Academy. The story begins with the sudden invasion of Kibi by the Galactic Empire, a powerful interstellar nation. The invasion leaves the kingdom in disarray, and the Amaterasu, initially a non-combat vessel, becomes one of the few remaining military assets capable of resisting the Empire.

The protagonist, Kio Kujo, is a young cadet and the daughter of Kibi's prime minister. Following the invasion, she assumes command of the Amaterasu, transforming it into a functional warship. The crew, composed primarily of fellow cadets, must adapt to their new roles as active combatants. The series explores their struggles as they navigate the challenges of warfare, leadership, and survival in a hostile galaxy. The Amaterasu's crew engages in a series of tactical battles against the Empire, utilizing their limited resources and ingenuity to outmaneuver a technologically superior enemy.

Key characters include Shinon Kujo, Kio's older brother and a seasoned officer who provides guidance and support; Risha Andou, the ship's chief engineer responsible for maintaining and upgrading the Amaterasu; and Annette Rosenheimer, a skilled pilot and Kio's close friend. The dynamics between the crew members highlight themes of camaraderie, responsibility, and the moral complexities of war.

The series delves into the political and strategic dimensions of the conflict, with the Amaterasu's crew broadcasting their battles to the galaxy in an effort to rally support for Kibi's resistance. This media strategy, combined with their tactical successes, gradually turns the Amaterasu into a symbol of hope and defiance. The narrative balances action-packed space battles with character-driven moments, emphasizing the personal growth of the crew as they confront the realities of war and their roles within it.