Count Butler serves as the primary antagonist of the third One Piece film, Chopper's Kingdom on the Island of Strange Animals. He is the leader of a trio of human hunters who terrorize Crown Island, accompanied by his two subordinates, General Hotdog and President Snake.

Count Butler is distinguished by his unusual practice of consuming animal horns, specifically seeking out horned beasts on the island. He plays the violin, and his music is used to incite and control the creatures he hunts, compelling them to charge and making them easier targets for his associates to cut down. His primary motivation is to acquire the island's sacred treasure, a legendary crown. He believes that by eating this crown, he will inherit immense powers capable of shaking the foundations of the universe.

In the story, the Straw Hat Pirates become separated after their ship, the Going Merry, is caught in a ring of geysers surrounding Crown Island. While Chopper is mistakenly proclaimed the new king by the island's talking animals, Count Butler and his men are already hunting the horned creatures to find the treasure. Butler's actions of extreme cruelty toward the animals and his goal to steal the island's sacred crown bring him into direct conflict with the Straw Hat crew, who ultimately move to stop him. There is no significant documentation regarding a character arc or development for Count Butler within the film, as he serves in the capacity of a villainous obstacle for the protagonists. His notable abilities include his violin playing, which directly influences the behavior of the island's animal population.