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In the first Dragon Ball film, Curse of the Blood Rubies, Kame Sennin appears as a legendary martial arts master who resides in a small house on a remote island. Also known as the Turtle Hermit and honored with the title Muten Rōshi, which means Old Master of Martial Arts, he is an elderly but extraordinarily powerful figure. His appearance is distinctive and iconic: a bald head, thick white mustache and beard, a tall walking staff, and a turtle shell worn on his back. He typically wears a red martial arts gi or casual beach clothing and is seldom seen without his round, tinted sunglasses.

The character is defined by a sharp and deliberate duality. On one hand, he is a wise and ancient master who teaches a rigorous philosophy of discipline, hard work, and the responsible use of power. He is the creator of the Kamehameha, a powerful energy wave technique, and his martial arts prowess is nearly unrivaled in the early part of the story. On the other hand, he is notoriously lecherous, often requesting to see or touch young women as a condition for his help, which provides much of the film's comedic relief. This foolishness, however, belies a deep sense of responsibility. He does not seek fame or glory and is content to live a simple life, only intervening when the natural balance is threatened.

In Curse of the Blood Rubies, his role is that of a reluctant but crucial mentor and a powerful ally. The main characters, Son Goku and Bulma, seek him out, initially hoping he can help them defeat the tyrant King Gurumes. Kame Sennin is initially more interested in Bulma than in their plight, but his heroic nature emerges when he witnesses the tyranny of the Gurumes Army firsthand. He demonstrates his incredible power by destroying a military submarine with a single, devastating Kamehameha wave, proving that his age has not diminished his strength. He explains that while he could solve their problems for them, true growth requires young warriors to face their own challenges. Consequently, he refuses to join their final assault but gives Goku his blessing and the Flying Nimbus cloud, a magical vehicle that only a pure-hearted person can ride, thereby validating Goku's innocence and potential.

His key relationships in the film are primarily with the younger generation. He acts as a surrogate grandmaster to Goku, immediately recognizing the boy's extraordinary talent and pure heart. With Bulma, his interactions are comedic and transactional, driven by his desires, though he never uses his strength to coerce her. The film does not feature his more famous students, Krillin or Yamcha, in prominent training roles. Instead, it establishes his connection to the wider world as a guardian who has trained past pupils, including the Ox-King, but now lives in semi-retirement.

He does not undergo a significant emotional transformation within the narrative of this specific film. He begins and ends as a static character, representing the archetype of the old master who tests the heroes before entrusting them with their destiny. The film serves to establish his foundational traits that will be explored in later stories: his incredible power, his perverted habits, and his profound wisdom.

Regarding his notable abilities, the most prominent is his signature technique, the Kamehameha. In the film, he demonstrates mastery over it, unleashing a wave of focused energy strong enough to annihilate a modern military submarine. He also possesses immense physical strength and speed that defy his aged appearance, along with centuries of combat experience. The official introduction of the character notes that he was the first person to climb the legendary Karin Tower centuries ago and is the inventor of the Kamehameha, a technique that took him fifty years to perfect. His longevity is attributed to a combination of martial arts mastery and consuming an enchanted herb.