Mizaru appears in the anime adaptation of "Last Period: the journey to the end of the despair" as part of the rival group Wiseman, opposing the main protagonists, the Arc End 8th Squad. Wiseman comprises three members: Mizaru, Kikazaru, and Iwazaru. Mizaru directly embodies the "see no evil" principle from the "Three Wise Monkeys" motif, visually expressed through her large hat covering her eyes.

Wiseman, including Mizaru, frequently interfere with the Arc End 8th Squad's quests and objectives, creating recurring obstacles. Their antagonistic role contributes comedic and competitive dynamics to the series. Mizaru and the other Wiseman members perform the anime's ending theme song, "Wiseman no Theme" (ワイズマンのテーマ).

In the original mobile game's unit roster, a character named Mizaru is a 5-star unit of the "darkness" attribute, classified as a "Defender" type from the "Unknown" realm; this gameplay role provides no narrative details for the anime character.