Anissina von Karbelnikoff, nicknamed Crimson Anissina for her long red hair typically swept into a high ponytail with curled ends, hails from one of Shin Makoku’s ten noble families, with her brother Densham leading the House of Karbelnikoff. As one of the Three Great Witches, she champions women's social empowerment, modeling this cause through her own actions. Her intellect and pragmatism fuel a bold, unapologetic demeanor—she openly acknowledges failed inventions and once slapped Maou Yuuri to provoke his focus, yet seamlessly adopts noble decorum when required. Though briefly considered a Maou candidate during a leadership void, this prospect dissolved upon Yuuri’s return.

An inventor, Anissina tags creations with descriptive names ending in "-kun," like the Mazoku-translating earplugs "Let Me Hear Your Heart's Cry-kun." She routinely tests these devices on apprehensive childhood friend Gwendal von Voltaire; if he’s unavailable, she recruits Günter or Wolfram—though Wolfram often escapes. Conrad von Weller refuses testing, lacking the maryoku essential for activation. Beyond engineering, Anissina taught Gwendal to knit and later instructed Greta, preserving his first knitted sand bear as a cautionary lesson piece.

She pens adventure tales casting herself as "Poison Anissina," a superheroine. While Greta delights in these stories, Wolfram and Yuuri debate their suitability for children. Additional facets include her fear of roosters (opposing Densham’s avian affection), Densham’s past scheme to wed her for financial gain, her tutoring of Lindsey von Wincott, her self-styled epithet "Poison Woman," and her deliberate choice to remain unmarried.