Ayane Mizuchi appears in the OVA series "Anata dake Konban wa" as one of the Mizuchi sisters alongside Mone Mizuchi. She attends the same school as protagonist Yoshisuzu Shougo and confronts him early in the narrative, declaring herself his fiancée due to a parental arrangement made without her initial consent. During their first meeting, she openly states she does not yet consider Shougo worthy of the engagement, immediately creating tension between them.

Ayane's popularity at school amplifies the situation; her engagement to Shougo triggers jealousy and resentment among other students, compounding the challenges he faces in his new environment. She operates within a group of female characters competing for Shougo’s attention, including maid Youko Otosumi, school nurse Sakurako Rokuhira, and spectral figure Reiko who haunts Shougo’s visions. Ayane’s interactions with these characters center on romantic and sexual tensions within the series’ harem structure.

No further details exist about Ayane Mizuchi’s background, family history, or personal development beyond her initial role. The documented material does not explore her evolution across episodes, motivations extending beyond the engagement conflict, or character growth. Her connection to the central mystery involving ghost Reiko—who resembles a portrait in Shougo’s mansion—remains unelaborated, as do relationships outside the harem dynamics or her individual narrative resolution.