M. Bison serves as the animated film's primary antagonist, commanding the criminal organization Shadaloo (known here as Shadowlaw) in his quest for global domination. He relentlessly pursues powerful fighters, deploying monitor cyborgs worldwide to identify skilled martial artists for abduction and brainwashing into his forces. Ryu stands as his most coveted target due to exceptional combat prowess, with Ken Masters becoming a secondary focus when Ryu proves elusive.

Bison exhibits a ruthless, arrogant personality, viewing others solely as tools for his ambitions. He openly enjoys inflicting suffering, exemplified by subjecting Ken to psychic torture to break his will and establish control. His imposing physical presence is marked by a military-style uniform and cape, reinforcing his authoritarian nature. In combat, he wields purple Psycho Power energy, enabling teleportation, energy projection, and enhanced physical attacks.

Personal conflicts define his interactions: Captain Guile hunts him for murdering friend Charlie, while Chun-Li seeks vengeance for her father's death. After capturing Ken in the United States, Bison transfers him to Shadaloo's base near the Thailand-Laos border. Upon locating Ryu, Bison travels there with the brainwashed Ken, intent on securing his ultimate prize.

Bison confronts Guile upon arrival and defeats him with ease. He spares Guile's life, leaving him to endure the humiliation of defeat, reflecting Bison's perceived superiority and contempt for opponents. Simultaneously, Ken fights Ryu under Bison's control until Ryu breaks the mental programming, freeing Ken.

Enraged, Bison attacks Ken with Psycho Power before engaging Ryu directly. He initially dominates the fight, showcasing superior power and technique. However, Ken recovers and rejoins Ryu. Though Bison effectively counters their combined assaults, sustained attacks eventually weaken him. The conflict culminates with Ryu and Ken defeating Bison using a simultaneous Hadoken attack that appears to destroy both him and his aircraft.

The film concludes with a post-credits scene showing Bison alive, operating a truck and attempting to run down Ryu walking along a road, implying his survival despite the battle, though its canonical status within the narrative remains ambiguous.