Alessandro, known alternatively as Alexander or Mousy in the Japanese version, appears solely in the second season of the 1975 anime adaptation. This bespectacled mouse embodies a highly intelligent and nerdy nature, coupled with a pronounced fondness for cheese. His personality blends intense intellectual curiosity with moments of social awkwardness, occasionally resulting in interactions perceived as blunt or rude by other meadow inhabitants. Despite this trait, he forges a strong bond with Maya and Willy, becoming one of their trusted companions.

Mutual respect and camaraderie define Alessandro's dynamic with Maya and Willy, central to his role. His close friendship with Maya, however, sometimes sparks jealousy in Willy, particularly when Maya focuses on Alessandro's intellectual insights or problem-solving skills. This tension marks their interactions but coexists with their underlying loyalty.

Within the group, Alessandro serves as a source of knowledge and alternative perspectives, frequently contributing logical or unconventional solutions to challenges faced by Maya and her friends in the meadow. His expertise proves valuable in navigating complex situations. His character remains consistent throughout his appearances, with no significant documented evolution.

Alessandro originates exclusively in the 1975 anime's second season and does not feature in Waldemar Bonsels' original 1912 literary work. Subsequent adaptations, including the 2012 CGI series and the Maya the Bee film franchise (*Maya the Bee Movie*, *Maya the Bee: The Honey Games*, *Maya the Bee: The Golden Orb*), also lack his presence.