Jane Pika becomes Patty Rabbit's primary guardian after Patty moves to Palm Town, responsible for her daily care and upbringing. She co-owns and operates the Pika Clinic with her husband, Dr. George Pika, conducting patient examinations and managing clinical operations. Jane shows significant dedication to medical duties but initially undervalues non-clinical tasks like cooking, leading to disagreements with George about clinic role priorities.

Her perspective shifts when she and George temporarily switch roles at the clinic, an experience facilitated by Patty and Rolley Cocker. Jane takes on kitchen duties while George handles patient care. This role reversal demonstrates her evolving understanding of diverse contributions to the clinic beyond her initial undervaluation. Her noted lack of culinary proficiency, described as "out of practice," contributes to practical challenges during this period.

Beyond the clinic, Jane provides familial stability for Patty in Palm Town, integrating her into the community and supporting her adjustment from Maple Town. Her relationship with Patty extends into collaborative problem-solving, as seen when she participates in the clinic role-swap at Patty's encouragement. Her background prior to Palm Town and extended family connections beyond her marriage remain undefined.