Dr. Nitro acts as Lord Zero's minion, tasked specifically with constructing specialized machines to locate and acquire valuable treasures. These inventions consistently malfunction or fail during operations. After each failure, Nitro habitually deflects responsibility using the phrase, "it wouldn't have happened if you gave me more time and money," establishing it as a defining verbal trait. Lord Zero reacts with particular disdain whenever this excuse is voiced.

Nitro's role remains focused solely on providing technological support for Lord Zero's treasure-hunting endeavors, despite the repeated shortcomings of his contributions. He shows no significant evolution or backstory development. His presence introduces comedic elements through the contrast between his proclaimed genius and his inventions' impractical results, alongside the recurring dynamic triggered by his catchphrase. He maintains this specific function throughout his appearances.