Ptilol emerges in the third season of the *Sailor Moon Crystal* anime during the Death Busters arc. She is born from Sailor Moon's Moon Spiral Heart Attack striking the witch Cyprine, splitting her into two distinct entities: Cyprine and her shadow counterpart, Ptilol.
Ptilol functions as an extension of Cyprine's power rather than a fully independent being, forming a single unit with her. Together, they serve as the fifth member of the Witches 5 within the Death Busters. Their power corrupts targets by drawing out the darkness within their hearts, mirroring the Amanojaku of Shinto-Buddhist lore.
In battle, Ptilol and Cyprine employ a coordinated strategy where one blocks attacks while the other strikes, creating a near-invincible defense and offense that challenges the Sailor Guardians. Ptilol meets her end when Sailor Moon transforms into Super Sailor Moon using the Holy Grail, channeling the combined energy of the Sailor Guardians. Super Sailor Moon's Rainbow Moon Heartache attack obliterates both Cyprine and Ptilol simultaneously.
Ptilol's entire existence, background, and development occur solely within the Death Busters arc of *Sailor Moon Crystal*.