Sophia Amae, an illegitimate child from the Tower's Middle Area, leveraged her exceptional intelligence to become an assistant researcher for the Workshop, specializing in Living Ignition Weapon projects. Her duties centered on supervising and educating test subjects Kang Horyang (No. 21) and Beniamino Cassano (No. 99), plus the later subject Beta. She taught them the universal Macsethian language and fundamental human interaction until age twelve, driven by experiments aimed at empowering abandoned and illegitimate children. Typically clad in a brown turtleneck, black dress, and scientist's coat, with brown eyes and brown hair, Sophia maintained a consistently cheerful demeanor. She treated Cassano and Horyang as humans, not subjects, contrasting other researchers like Maddox. She named Horyang "Beniamino Ilmar" and Cassano "Beniamino Cassano," replacing their numbers. Her activities—talking, laughing, playing—created deeply meaningful experiences Horyang later recalled. Years later, aboard the Archimedes airship within the Acorn Workshop during the Workshop Battle arc, Sophia resumed her Living Ignition Weapon work on Beta and Emile. Her involvement with new subjects prompted Horyang to question the sincerity of her past affection. She alerted guards to Beta's escape and intervened when Beta attacked the Irregular Viole, clarifying after Beta's defeat that research targeting Viole was separate from Beta's actions. Sophia tended Beta's injuries inflicted by Workshop guards. She pleaded with Ranker Lero-Ro to take Beta away, advocating for his freedom in his remaining time. She provided critical information: Horyang's coma resulted from Cassano removing the devil within him, and Emile's true nature as an Ignition Weapon. Sophia affirmed the sincerity of her care for Horyang and Cassano, expressing concern for them, Beta, and Viole, urging protection from exploiters. Her words to Horyang emphasized resilience: "Listen, Ilmar. A man shouldn't show his tears. You should be strong enough to protect others. You have that power. Got it?" Horyang described her as his and Cassano's first love.