A divine being from Greek mythology, Aphrodite enlists five schoolgirls as Venus Warriors to protect Earth. She secretly selects each warrior, marking them with a unique Venus symbol hidden on their bodies. Her primary foe is the Inma Empire, commanded by Necros, whose goal is to resurrect Apollo, the god of lust, and reshape the world into a sexually dominated realm.

Aphrodite operates remotely, dispatching her feline servant Buccha to locate the chosen girls and reveal their destinies. To access supernatural combat abilities, the warriors undergo transformation. While Aphrodite provides strategic oversight, her guidance is indirect, channeled through Buccha. She maintains a detached and occasionally condescending attitude towards her recruits.

Her core motivation is thwarting the Inma Empire's conquest, positioning her as a celestial guardian. However, her involvement remains strictly strategic, avoiding direct battle intervention. The conflict climaxes as the Venus Warriors confront the Inma Lords—faculty members at the warriors' school in disguise—who employ tactics to uncover the warriors by detecting their concealed symbols.

Aphrodite's role is confined to that of an orchestrator. Her character remains defined by this function, with no significant evolution beyond her initial portrayal across the series' two-part narrative.