In the anime "Mask of Zeguy" (or "Unkai no Meikyū Zeguy"), Leonardo Da Vinci appears as a fictional reimagining distinct from his historical counterpart. He is an inventor singularly obsessed with creating destructive weaponry, prioritizing this pursuit above all else. Driven by a craving for recognition, he allies with Sokatsuryou Koumei against Himeko because Sokatsuryou values Leonardo’s destructive inventions while Himeko dismisses them. Though initially indifferent to their conflict over the Gate of Winds, his support remains pragmatic and self-serving.

Later, he attempts to seize the "bell" artifact personally to ensure direct control over outcomes. His inventions include cyborg warriors like Kuan Yu and Chouhi, yet he strongly prefers constructing high-impact cannons. A unique feature is his miniature marionette replica, physically attached to a harness or frame, which mirrors his gestures and speech in real time; this smaller figure is explicitly non-mechanical.

Aligned with antagonists Himiko and Zhuge Liang in the parallel dimension of Unkai, he seeks collective domination of that world. His character undergoes a "Historical Villain Upgrade," transforming him into a figure who tests weapons on living subjects—contrary to historical accounts suggesting the real Leonardo may have sabotaged his military designs to prevent warfare.