Misako Takashina serves as an officer for the international defense organization Spirits. She recruits protagonists Kei Anno and Naoki Matsudo, presenting Spirits as an alternative schooling option while concealing its true purpose as a military pilot training program. After securing their enrollment, she facilitates their relocation to a specialized training facility alongside other selected candidates.

Takashina's responsibilities include operational oversight during missions involving the Devadasy mecha unit. When Kei exhibits altered, aggressive behavior under the Devadasy nanomachine influence, she intervenes directly, seducing him to obtain a sperm sample for analysis. This action confirms his nanomachine infection. Later, during an alien infection crisis at the Spirits base, she collaborates with officer Yamada to activate the facility's self-destruct protocol. This sacrifice contains the threat and disrupts an ongoing alien absorption process.

Her background prior to joining Spirits, familial connections, and personal motivations beyond her operational duties remain undocumented. The character appears exclusively within the original OVA episodes, without significant personal development or resolution of her storyline.