Chiyoko Wato appears as a minor character in "Tezuka Osamu ga Kieta?! 20 Seiki Saigo no Kaijiken," portrayed as a self-confident, tomboyish high school student. Her principal narrative role stems from "The Three-Eyed One" series, where she serves as Sharaku's friend and guardian. Crucially, only she possesses the ability to apply and remove the bandage sealing Sharaku's third eye, controlling his transformation and preventing the release of his dangerous mystical powers.

Within the broader Tezuka universe, the "Black Jack" TV series depicts Chiyoko as Sharaku's elder sister. In this familial dynamic, she frequently nags him about developing greater self-confidence and completing his homework, reflecting her protective and supervisory nature.

During the New Year's Eve 2000 celebration at Dr. Tenma's mansion in "Tezuka Osamu ga Kieta?! 20 Seiki Saigo no Kaijiken," Chiyoko participates in the gathering of Tezuka's characters. When Tezuka Osamu vanishes mysteriously, she joins fellow characters in investigating and resolving the incident. Her inclusion in this special aligns with her established role across Tezuka's interconnected character system.