Chuzaemon Makuya is a character from the 1972 film Lone Wolf and Cub: Baby Cart at the River Styx, portrayed by actor Seishirō Hara. His primary role in the narrative is that of a target, serving as the central figure in the assassination contract that drives the film's plot. He is identified as a traitor to the Awa clan, as he intends to sell the clan's profitable secret method for producing indigo dye to the Shogunate. This act of betrayal marks him as a significant threat to his clan's economic security and social standing, leading the elders of the Awa clan to hire the master assassin Ogami Itto to eliminate him.

Information regarding Chuzaemon Makuya's background prior to these events, his personality, and his motivations beyond his act of treason is not available. His role in the story is fundamentally that of a quarry. To protect him on his journey after his betrayal, he is placed under the escort of three formidable warriors known as the Hidari brothers, who are collectively referred to as the Gods of Death. His relationships with these bodyguards are purely transactional, as they have been employed to ensure his safe passage. His character does not exhibit notable development within the film, as his narrative function is to be pursued and ultimately confronted by the protagonist, Ogami Itto. No specific combat skills or unique abilities are detailed for Chuzaemon Makuya, as his significance in the story derives from his political and economic value as a defector rather than from any personal prowess.