Episode 2 focuses on Giyuu Tomioka, the Water Hashira, and showcases his inner struggles. Giyuu does not believe he is worthy of being a Hashira, as he feels unworthy due to his past. During the final selection, Giyuu survives without killing a single demon, as he spends most of the test unconscious after injuring himself. His friend Sabito, who had been trained by Sakonji Urokodaki, died while trying to protect others, including Giyuu. Had Sabito survived, he would have become the Water Hashira in his place.

Giyuu's self-doubt is amplified by the current emphasis on awakening special marks, which he believes he cannot achieve. He sees himself more as a replacement rather than a true Hashira.

The episode also discusses the significance of Giyuu's haori. The brown half comes from his sister, who was killed by a demon while protecting him, and the patterned half comes from Sabito. This haori symbolizes the memory and protection of his loved ones and reminds Giyuu of his duty to protect others.

Kanao Tsuyuri makes a brief appearance, as the episode coincides with her birthday. Meanwhile, Shinobu Kocho does not participate in the training but instead focuses on researching Nezuko's ability to resist the sun alongside Tamayo. Shinobu's anger hints at unresolved issues, and she plans to tell Kanao about the demon who killed her older sister and how to kill it.

The demon who killed Shinobu's sister is still alive, and Shinobu knows how to kill it. However, she has not yet had the opportunity to do so. There is speculation that Tamayo might be that demon, which would explain Shinobu's anger and the Demon Slayer Corps' interest in her research. Shinobu is preparing Kanao to face the demon.