Toshie Negishi is a Japanese actress primarily known for her extensive work in live-action film and television, with a career spanning from the 1970s to the present. She was born on February 3, 1954, in Tokyo, Japan. She studied drama at Toho Gakuen College but left before completing her degree. Throughout her long career, she has been affiliated with the talent agency Alpha Agency.

Negishi's filmography is substantial, encompassing numerous roles in acclaimed and diverse films. Some of her notable live-action film credits include appearances in director Akira Kurosawa's Dreams in 1990 and Rhapsody in August in 1991. She has also worked with prominent directors like Takashi Miike, appearing in Audition in 1999. Her other film appearances include The Sea and Poison in 1986, Sada in 1998, and 100 Yen Love in 2014. Her television work is equally extensive, with roles in series such as The Queen's Classroom in 2005 and the taiga drama Idaten in 2019.

While her career is overwhelmingly centered on live-action performance, Negishi has contributed her voice to anime. Her most prominent voice acting role is Setsu Ōiwa in the 2014 Studio Ghibli animated film When Marnie Was There. In this film, she voices the kind and patient wife of Kiyomasa Oiwa, who, along with her husband, cares for the protagonist Anna during a summer in a rural town. This role represents a notable, though singular, entry into anime voice acting within a career defined by on-screen performances. Information on recurring collaborations with specific anime directors or studios is limited, as her voice work in animation appears to be infrequent.

Negishi's notable achievements are primarily in the realm of live-action cinema and television, where she has built a reputation as a prolific and enduring character actress. Her decades-long career and collaborations with many of Japan's most celebrated filmmakers stand as a testament to her standing in the industry. Her role in When Marnie Was There connects her to the legacy of Studio Ghibli, marking her sole, but significant, foray into animated feature films.