Ai Matayoshi is a Japanese voice actress from Osaka Prefecture, born on April 2, 1983. She is affiliated with the talent agency Arts Vision. Her interest in becoming a voice actor was sparked during her junior high school years through playing the game Tokimeki Memorial. She pursued this goal by training at the Japan Narration Acting Institute before joining her agency.

Matayoshi began her career in the early 2000s, with some of her earliest anime roles appearing in 2004. Over the years, she has built a extensive list of credits across anime, video games, and drama CDs. In anime, she is known for a range of characters, including Kobato Haneda in We Without Wings - Under the Innocent Sky, Miriam Polk in Horizon in the Middle of Nowshere, and Hinata Kudō in the OVA Otome wa Boku ni Koishiteru: Futari no Elder. Other notable anime roles include Satoka Mundo in the Saki series, Suzu Azuma in Yozakura Quartet, and Yutaka Imai in A Channel. In the realm of video games, she has had prominent roles such as Nao in the Dream Club series and Alice in Criminal Girls.

Throughout her career, Matayoshi has engaged in several recurring collaborations. She was a member of the voice acting unit Puppy's alongside Ito Yasuko and Hasegawa Shizuka, and she also formed another unit, Hapi Raba (Happy Lover), with fellow voice actress Kadota Sachiko. Her work in the Dream Club game series, providing the voice for the character Nao, represents a long-running role in a single franchise.

Notable achievements in her career include voicing main or recurring characters in multiple television series and their subsequent adaptations, such as her roles in Yozakura Quartet and A Channel, which continued across OVAs years after the initial TV broadcast. Her performance as Suzu Azuma, for example, spanned multiple TV series and OVA adaptations. Her work is recognized across various media, demonstrating her versatility and continued presence in the industry from the 2000s through to the 2020s.