Charlotte Ann is a professional voice actor based in Los Angeles, known for her work in animation and video games. She was born in the Bronx, New York, and moved to Columbus, Ohio, at the age of eight. Her early interest in the performing arts was fostered at Fort Hayes Performing Arts High School in Columbus, where she studied theatre and music, playing the trumpet. She continued her training at the University of Southern California's School of Theatre, graduating cum laude. After initially working in stage and on-screen acting, she transitioned to focus on a career in voiceover.

Her voice acting credits include a range of characters in animation and video games. She provided the voice for the character Double in the fighting game Skullgirls, a role she was approached for by the game's audio director. In a notable achievement, she voiced Princess Styla in the Nintendo game The Legend of Zelda: Tri Force Heroes, marking the first time a princess in the main series of the franchise was given a speaking role in that era. She also voiced Patsy Walker in the mobile game Marvel Avengers Academy. Her film work includes the role of Konohana in the English version of the animated feature The Mystical Laws.

Throughout her career, Ann has engaged in recurring collaborations with industry professionals. She worked with voice director and fellow actor Cristina Vee on the Skullgirls project. Her husband, Chris Riffey, a tech and game design professional, has been a significant collaborator in building and maintaining her home recording studio, which is essential for her voiceover work.

Ann's performance as Princess Styla for Nintendo represents a significant credit in a major international franchise. Her body of work has been recognized with several industry accolades, including a Telly Award, a Reed Award, and two Society of Voice Arts and Sciences Awards. Beyond character work, her voice is featured in commercial campaigns for major brands such as Apple, Cinemark, LEGO, Subway, Target, and Whole Foods. She is also a member of the Angel City Chorale, which competed on the television program America's Got Talent.