Yui Tsukada is a Japanese voice actress affiliated with the agency I'm Enterprise. She was born on June 5th and is a native of Kyoto Prefecture. Her career began to take shape after she won a special award at the third Voice Actor Soul competition in Kyoto in 2014. This recognition led to her role as Saki Matsuga, the character for a promotional project by the Kyoto City Transportation Bureau, which was part of the Chikatetsu ni Noru initiative. Following this, she trained at the Japan Narration Acting Institute. She became an official member of I'm Enterprise in April 2020.

In anime, Yui Tsukada has built a portfolio of roles, often lending her voice to young boys, children, and students. Her television anime credits include appearances in series such as The Maid I Hired Recently Is Mysterious, Dropkick on My Devil! X, The Magical Revolution of the Reincarnated Princess, Undead Unluck, and Wind Breaker. She also voiced the character Transcend in the Uma Musume Pretty Derby franchise, appearing in both the game and the 2024 theatrical film Uma Musume Pretty Derby: Beginning of a New Era. She has provided voices for various video games, including AFK Arena.

Her first leading role in an anime film was announced in 2025, when she was cast as the protagonist Musashi in the original theatrical anime KILLTUBE. Beyond animation, her work includes dubbing for foreign dramas and films, as well as voice roles in audio dramas and有声漫画.