Jared Padalecki is an American actor best known for his live-action roles, particularly as Sam Winchester in the long-running television series Supernatural. His work in anime voice acting is defined by a single, significant role that directly connects to his live-action career.

Padalecki provided the English voice for the character Sam Winchester in the 2011 anime adaptation, Supernatural: The Anime Series. This 22-episode series, produced by the renowned Japanese animation studio Madhouse, retold and expanded upon the storylines from the first two seasons of the live-action television show. For this project, Padalecki reprised his iconic role, voicing Sam in every episode of the animated series. This commitment made him the only original cast member from the live-action series to voice his character for the entirety of the anime's run, as Jensen Ackles, who played Dean Winchester, was only able to voice the role for the final two episodes due to scheduling conflicts. The English-language production also featured Andrew Farrar as the primary voice of Dean for the first 20 episodes.

The Supernatural anime series was first released in Japan in early 2011 and subsequently in North America on DVD and Blu-ray in July of the same year. Padalecki's participation extended beyond just voice work; he and Jensen Ackles also provided brief introductions for each episode on the home video release.

This role as Sam Winchester in Supernatural: The Anime Series stands as Padalecki's sole credit for anime voice acting. His career remains primarily focused on live-action television and film, making this project a unique and notable intersection of his most famous part with the medium of animation.