Mahershala Ali, widely recognized for his live-action performances, has also developed a significant career as a voice actor in animation. Among his notable roles is the character Vector in the 2019 film "Alita: Battle Angel," an adaptation of the manga series "Gunnm" by Yukito Kishiro. In this film, he portrays Vector, a villainous figure who oversees and fixes matches in a gladiator-style combat game known as Motorball. The role was a notable entry point for Ali into the world of animated and motion-capture performances.

Beyond "Alita: Battle Angel," Ali has lent his voice to several prominent characters in American animated productions that have strong ties to comic book and superhero genres, which are often closely associated with anime fandom. He voiced Aaron Davis, also known as the Prowler, in the critically acclaimed animated films "Spider-Man: Into the Spider-Verse" (2018) and its sequel "Spider-Man: Across the Spider-Verse" (2023). His performance as the character brought emotional depth and moral complexity to the role, contributing to the films' widespread success. He is expected to reprise this voice role in the upcoming film "Spider-Man: Beyond the Spider-Verse".

Another major voice role for Ali is Titan in the adult animated superhero series "Invincible," which premiered in 2021. This series is known for its graphic violence and complex characters, and Titan is a memorable figure within its storyline. Ali has also ventured into voice work for video games, providing the voice for the character Cutter Wade in the "Longshot" story mode of "Madden NFL 18". Additionally, he has an upcoming voice role in the animated film "Wildwood," scheduled for release in 2026.

Ali's voice acting career is characterized by his recurring collaborations with major studios such as Sony Pictures Animation and Amazon Studios. His work in the "Spider-Verse" films represents a long-term partnership with Sony, portraying different versions of the same character across multiple installments. His role in "Invincible" places him within an ensemble cast for Amazon Prime Video. Outside of his voice work, his achievements as a performer include winning two Academy Awards for Best Supporting Actor for his roles in "Moonlight" and "Green Book," as well as a Primetime Emmy Award as a producer, highlighting a career marked by critical acclaim and recognition for his artistic range.