Milo Ventimiglia has a focused but notable portfolio as an anime voice actor, centered primarily on his role as the English dub voice of Wolverine, also known as Logan, in the Marvel anime projects produced in collaboration with the Japanese studio Madhouse. This work represents a specific chapter in his broader career as an American television and film actor.

Ventimiglia was announced to voice the title character in the 2011 anime series Wolverine by Marvel's Head of Television, Jeph Loeb, at the WonderCon comic convention in San Francisco. The series was part of a larger collaboration between Marvel and Madhouse that produced anime adaptations of several Marvel properties, which aired on the G4 cable network in the United States. His casting alongside fellow Heroes actor Adrian Pasdar, who voiced Iron Man, was highlighted for bringing stars from a popular live-action superhero drama to the voice cast of these animated projects.

In the Wolverine anime, Ventimiglia takes on the lead role of Logan, a character with a long and iconic history in comics and other media. The series follows Logan as he travels to Japan to find his kidnapped girlfriend, Mariko Yashida, leading him into conflict with the criminal underworld. In an interview about his work on the series, Ventimiglia described the process of finding his own interpretation of Wolverine's voice, aiming to make it sound natural and distinct while paying homage to previous versions of the character. He noted that the unique animation style of the Japanese production gave him room to play Logan as a somewhat younger and occasionally more humorous version of the character while still maintaining the necessary edge and anger.

Beyond his starring role in the twelve-episode Wolverine series, Ventimiglia also reprised the role of Logan for guest appearances in other entries of the Marvel anime lineup. He provided the voice for the character in an episode of Marvel Anime: Iron Man and again in an episode of Marvel Anime: Blade. However, he did not voice Wolverine in the related Marvel Anime: X-Men series; for that production, the role was performed by Steven Blum, a veteran voice actor known for his long-standing portrayal of the character across numerous projects. This was reportedly because the X-Men anime featured an older version of Wolverine.

While Ventimiglia has voiced comic book characters in other animated projects, such as Green Arrow on the stop-motion series Robot Chicken and Spider-Man Noir on Ultimate Spider-Man, these are not considered anime productions. His work in the Marvel anime series of 2011 remains his most significant contribution to the field of anime voice acting and a distinct part of his career.