Ami Matsushima is a Japanese voice actress affiliated with the agency Aoni Production. She is from Fukui Prefecture and her birthday is November 8. Her Japanese name is written as 松嶌杏実. She became interested in pursuing voice acting as a career after watching the character Yubaba from the film Spirited Away.

Matsushima has built a career with numerous supporting roles in long-running anime series and popular video games. Her voice acting work began in the 2010s. In television animation, she has played guest or recurring characters in major franchises such as One Piece, Doraemon, Chibi Maruko-chan, GeGeGe no Kitaro, and the sixth installment of the Pretty Cure series. She had a recurring role as multiple characters including a beautician and Yukidarumon in Digimon Ghost Game from 2021 to 2022. She also provided voices for several characters in the series Escape Great Mission. In the series Digimon Beatbreak, she voices the character Sapotama, an egg-shaped artificial intelligence device that serves as a central tool for the characters.

Beyond anime, Matsushima has an extensive portfolio in video games. Her game credits include roles in major titles such as Persona 5 Royal, Persona 3 Reload, and Xenoblade Chronicles X: Definitive Edition. She is also credited with voicing a character in Ninja Gaiden 4. Her dubbing work for foreign films and series includes providing the Japanese voice for characters in projects like The Iron Claw, Damsel, and the animated series Hazbin Hotel. Her other professional activities include narration for television programs and radio dramas. Her listed hobbies include visiting cafes and collecting cosmetics, and her special skill is singing.