"The Lusty Ladies of Mayohiga" is a comedic and ecchi anime series centered around the eccentric inhabitants of the fictional village of Mayohiga. The village is isolated from the outside world and populated almost entirely by women, each with distinct personalities and quirks. The narrative begins when a young man named Taro, an ordinary college student, stumbles upon Mayohiga after getting lost during a hiking trip. Upon his arrival, he becomes the center of attention for the village's female residents, who are both fascinated and flustered by the presence of a man in their midst.

The village is governed by a matriarchal system, with the wise but mischievous elder, Obaa-sama, overseeing its affairs. She sees Taro's arrival as an opportunity to bring change to the village and assigns him the task of helping the women adapt to the idea of male interaction. However, Taro's presence quickly disrupts the village's routine, as the women compete for his attention in increasingly absurd and humorous ways. The series explores the dynamics between Taro and the villagers, highlighting their misunderstandings, cultural clashes, and comedic misadventures.

Key characters include Hana, a shy and reserved girl who develops a crush on Taro; Yumi, a bold and confident woman who takes charge of situations; and Aoi, a tomboyish and athletic villager who initially resents Taro's presence but gradually warms up to him. Each episode focuses on a different villager or group of villagers, showcasing their interactions with Taro and their attempts to navigate their newfound feelings and curiosity about the outside world.

The plot is episodic, with each installment presenting a self-contained story while contributing to the overarching narrative of Taro's integration into Mayohiga. Themes of cultural exchange, personal growth, and the clash between tradition and modernity are explored through lighthearted and often risqué humor. The series balances its comedic tone with moments of sincerity, as the characters learn to understand and appreciate one another despite their differences. The setting of Mayohiga, with its lush landscapes and traditional architecture, serves as a backdrop for the series' playful and whimsical tone.