"Green Eyes: Ane Kyun! yori The Animation" is a short-form anime series that centers on the relationship between a young man named Yuuto and his older sister, Aneko. The story explores themes of familial bonds and romantic tension, set within a slice-of-life framework. Yuuto, a high school student, lives with Aneko, who is significantly older and serves as a maternal figure in his life. Their dynamic is complicated by Aneko's playful yet overbearing nature, as she frequently teases Yuuto while also caring for him deeply. The narrative delves into their daily interactions, highlighting moments of humor, awkwardness, and emotional vulnerability.

The plot unfolds through a series of vignettes that depict the siblings' lives, often focusing on Aneko's attempts to assert her dominance over Yuuto in both domestic and personal matters. These scenarios range from mundane activities like cooking and cleaning to more intimate moments where the boundaries of their relationship are tested. Aneko's flirtatious behavior and Yuuto's reluctance to engage create a recurring tension that drives much of the series' narrative. Supporting characters, such as Yuuto's classmates and Aneko's friends, occasionally appear to provide additional context or comedic relief, but the focus remains primarily on the central sibling relationship.

The series balances lighthearted comedy with subtle emotional undertones, portraying the complexities of familial love and the blurred lines between sibling affection and romantic attraction. The characters' interactions are often exaggerated for comedic effect, but the underlying themes of dependency, care, and emotional connection remain consistent throughout. The narrative does not delve deeply into dramatic conflict or resolution, instead opting for a more episodic structure that emphasizes character dynamics and situational humor. The result is a concise exploration of the nuances of sibling relationships, framed within a comedic and occasionally suggestive tone.