"Anime Tenchō" is a comedic slice-of-life anime that centers around the daily operations of a small anime shop and its quirky staff. The protagonist, Anime Tenchō, is the store manager, a passionate and eccentric individual deeply immersed in otaku culture. His encyclopedic knowledge of anime, manga, and related merchandise makes him both a resourceful leader and a source of humor, as his enthusiasm often leads to exaggerated or absurd situations. The shop itself serves as a hub for various otaku customers, each with their own unique personalities and interests, creating a vibrant and chaotic atmosphere.

The narrative unfolds through episodic vignettes, focusing on the interactions between Tenchō, his employees, and the customers. The staff includes a diverse cast of characters, such as the shy but dedicated clerk, the overly enthusiastic part-timer, and the cynical yet knowledgeable coworker. Their dynamics drive much of the humor, as they navigate the challenges of running a niche business while dealing with the eccentricities of their clientele. Common scenarios include managing limited-edition merchandise releases, handling obsessive fans, and participating in otaku events like conventions and cosplay gatherings.

The series also incorporates meta-humor, frequently referencing real-world anime and manga titles, industry trends, and otaku culture. These references are often exaggerated for comedic effect, highlighting the absurdity and passion inherent in fandom. Despite its comedic tone, the show occasionally touches on themes of community and belonging, as the shop becomes a gathering place for like-minded individuals who find camaraderie in their shared interests.

"Anime Tenchō" balances its humor with a lighthearted exploration of otaku life, offering a relatable yet exaggerated portrayal of the challenges and joys of running a specialty store. The episodic structure allows for a variety of comedic situations, ensuring that each episode feels fresh while maintaining a consistent tone. The characters' growth, though subtle, is evident as they learn to navigate their roles within the shop and their relationships with one another, creating a sense of continuity throughout the series.