"23-ji no Saga Meshi Anime" centers on the daily life of a salaryman named Sōta Nakahara, who works late shifts at a company in Saga Prefecture. After long hours at work, Sōta finds solace in exploring the local food scene during the late-night hours, often visiting small eateries, izakayas, and food stalls that remain open past midnight. The series highlights his culinary adventures, showcasing the unique dishes and regional specialties of Saga, while also delving into the personal stories of the people he meets along the way.

The narrative is episodic, with each installment focusing on a different dining experience and the interactions Sōta has with the proprietors and fellow patrons. These encounters often reveal the cultural and historical significance of the food, as well as the personal connections people have to their local cuisine. The series emphasizes the warmth and community found in these late-night gatherings, portraying food as a bridge between individuals and a reflection of the region's identity.

Sōta is portrayed as an ordinary, relatable protagonist, whose curiosity and appreciation for food drive the story. His character is grounded and unpretentious, making him an effective guide for the audience as he explores Saga's culinary landscape. The supporting cast includes a variety of shop owners, chefs, and other customers, each contributing their own perspectives and anecdotes, enriching the narrative with diverse voices and experiences.

The series blends slice-of-life storytelling with a culinary focus, offering a detailed and affectionate portrayal of Saga's food culture. It balances lighthearted moments with deeper reflections on tradition, community, and the role of food in everyday life. Through its episodic structure and emphasis on local cuisine, "23-ji no Saga Meshi Anime" provides a heartfelt and immersive exploration of a lesser-known region of Japan, celebrating its flavors and the people who bring them to life.