"Pui Pui Molcar Driving School" is a spin-off of the popular stop-motion anime series "Pui Pui Molcar," which features guinea pig-like cars known as Molcars. The series shifts focus to a driving school setting, where the Molcars learn the rules of the road and proper driving etiquette. Each episode centers around the Molcars attending lessons taught by their instructor, a humanoid guinea pig named Sensei. The lessons are designed to teach the Molcars essential driving skills, such as obeying traffic signals, navigating intersections, and parking, while also emphasizing safety and responsibility.

The Molcars, with their distinct personalities and quirks, often find themselves in humorous and chaotic situations during their lessons. Potato, the curious and energetic Molcar, frequently takes the lead, while others like Cabbage, Shroom, and Teddy contribute their unique traits to the mix. The driving school environment serves as a backdrop for both educational content and lighthearted comedy, as the Molcars' antics often lead to unexpected challenges for Sensei.

The series maintains a simple, episodic structure, with each episode focusing on a specific driving-related scenario. The narrative is driven by the Molcars' interactions with each other, their instructor, and the driving school environment. Despite the comedic tone, the series subtly reinforces themes of teamwork, perseverance, and the importance of following rules. The blend of educational elements and humor makes it accessible to a wide audience, particularly younger viewers, while retaining the charm and creativity of the original "Pui Pui Molcar" series.