Chief Makina, whose full name is Kiwako Makina, serves as the chief guard and chief warden of Deadman Wonderland. She is a woman in her mid-twenties with long, dark-brown hair, one lock of which often hangs beside her face, and dark blue eyes. She typically wears a dark blue prison uniform complemented by a matching hat and white pants, along with black knee-length boots that feature high heels. She is known for carrying a decorated katana, which she uses to enforce discipline within the prison.

Makina presents herself as a harsh and stone-cold enforcer who does not hesitate to punish prisoners, often brutally, for breaking the rules of the facility. Her actions are swift and severe, intended to maintain order through fear and immediate consequences. Despite this brutal exterior, her personality is not without nuance. She is known for being incredibly honest, sometimes in unexpected ways, once casually answering a prisoner's lecherous question regarding her breast size and discussing her diet with a subordinate in the middle of a serious situation. While she is a fierce disciplinarian, she is far less twisted and corrupt than other authority figures within the prison, particularly Tsunenaga Tamaki. She harbors deep suspicion towards him, feeling that her actual authority over Deadman Wonderland is limited and that many sinister operations are happening beyond her control.

Makina's primary motivation stems from a dedication to her duty and a growing distrust of the secrets being kept from her. As she begins to uncover the truth about the Wretched Egg, the Deadmen, and the artificial Deadmen known as Forgeries, her sense of justice compels her to act. Once she discovers Tamaki's true agenda, she abandons her post as chief warden and dedicates herself to a mission to defeat those orchestrating the facility's horrors. Her role in the story evolves from being an imposing symbol of the prison's oppressive system to a key ally who aids in exposing its atrocities.

Her key relationships include her subordinates, who demonstrate a strong devotion to her; when she resigns her position to pursue Tamaki, many of her guards choose to join her rebellion. She also has ties to the Special Forces from her past, where she learned her swordsmanship skills and befriended individuals like Eko Kaido, Kashima Toraichi, and Bonbu. Her relationship with the protagonist, Ganta Igarashi, begins on a hostile footing as she demonstrates the cruel reality of the prison to him, but she later becomes a crucial ally in his fight against the same forces.

Throughout the narrative, Makina undergoes significant development. She transitions from a loyal, albeit brutal, cog in the prison machine to a renegade leader fighting against the corruption she once unknowingly served. After discovering Tamaki's secrets, she initiates a coup d'état within Deadman Wonderland. She obtains a special sword embedded with Worm Eater technology, which is effective against the Forgeries, and uses it in battle. After being injured in a major conflict, she is confined to a wheelchair for a time. However, she returns to the front lines, ultimately playing a part in the final operations against the primary antagonists.

In terms of abilities, Makina is a highly skilled swordswoman, a talent she honed during her time in the Defense Force. Her primary weapon is her distinctive katana, which she wields with lethal precision. Later, she acquires a more advanced sword embedded with a substance known as Worm Eater, a weapon designed to counter the abilities of the Deadmen and Forgeries. Her leadership skills are also notable, as she commands the respect and loyalty of the prison guards, many of whom follow her even after she turns against the prison's administration.