Mitsuhide Akechi recurs throughout the anime series and its related media. Within the comedic structure, he serves as a retainer under Oda Nobunaga, enduring persistent mistreatment and humiliation. This dynamic establishes him as the perpetual target of practical jokes and hazardous tasks orchestrated by Nobunaga and other Oda faction members, particularly Mori Ranmaru, who frequently engineers compromising situations for him. The narrative suggests this ongoing victimization and role as the clan's "butt-monkey" fuels his motivations for eventually rebelling against Nobunaga. However, his betrayal attempts remain consistently inept and unsuccessful within the parody context, typically foiled before achieving meaningful impact. His storylines culminate in failure and further comedic suffering.

This core role persists across installments. He features in the original OVA, where his characteristic struggles and foiled rebellion appear among comedic sketches involving historical figures. His presence continues in the first television season, *Tono to Issho: Ippunkan Gekijou*, specifically in Episode 8, which spotlights the Oda household's eccentricities and Mitsuhide's experiences. The second season, *Tono to Issho: Gantai no Yabō*, further explores Oda household dynamics in Episodes 3 and 4, sustaining his role as a beleaguered subordinate subjected to Nobunaga's whims. Azai family episodes, structured as visual novel or text adventure segments, also include him, upholding his established pattern of misfortunes within the series' absurd humor. His portrayal remains consistent throughout, emphasizing comedic victimization and thwarted ambitions without significant deviation or deeper evolution.