Pamie, a four-year-old little blue penguin, resides in an Australian village. She is the twin sister of Nick Penguin and has three younger triplet brothers, indicating a close-knit family. Her physical traits include a yellow beak without visible nostrils. She consistently wears a red hair bow, a pink and red-striped scarf, and brown shoes across all weather conditions.

Her personality centers on pronounced enthusiasm for eating, which becomes a vulnerability when Walter Kangaroo’s teasing about her stomach size triggers temporary anorexia in the episode "Balloon Pamie," causing her to faint and highlighting her sensitivity to criticism. She harbors unrequited romantic feelings for Roobear Koala, driving narrative developments like "Pamie Falls in Love," where her brother Nick orchestrates a failed scheme to replace Roobear’s picnic date Betty Koala. After the plan collapses, she consumes the entire picnic herself, illustrating her emotional coping mechanism tied to food.

Pamie aspires to become a nurse, an ambition explored in episodes like "Nurse Pamie," reflecting her nurturing side. Her village friendships include Roobear, Floppy Rabbit, Mimi Rabbit, Laura Koala, and others, integrating her into the predominantly koala-inhabited community. Her family dynamics show awareness of her triplet brothers’ challenging behavior, as seen in "Roobear the Babysitter," where she acknowledges the difficulty of managing them.

A cosmetic mishap occurs in "Editor-in-Chief Roobear" when she interprets maternal advice about maturity as an instruction to use her mother’s makeup, leading to reprimand and demonstrating her occasional literal interpretation of guidance. Her character evokes sympathetic portrayals during narratives involving unreciprocated feelings or bullying, cementing her as an emotionally resonant figure within the series.