Anzelotte coordinates missions as the authoritative leader of the Night Wizards, operating from a castle either floating above Earth or in another dimension. She dispatches wizards to combat threats like Emulators and Dark Lords, mandating mission acceptance with the phrase: "For the request that I will ask you now please reply 'Hai' or 'Yes'"—eliminating refusal as an option. Renji Hiiragi faces particular enforcement, frequently summoned against his will even during school attendance.

Her interactions with Renji involve deliberate humiliation and torment. While transporting other Night Wizards comfortably, she retrieves him via methods like a giant helicopter hook. She disregards his discomfort or personal goals, prioritizing mission completion above all else, including publicly announcing his academic failures over school speakers. Toward others, she maintains an all-knowing, authoritative demeanor.

Visually, Anzelotte appears as a young lady with cyan-gray hair, violet eyes, and fair skin. She wears a dark renaissance gown with a white lace petticoat, a white lace ribbon collar featuring a red gem, white lace cuffs, gray tights, dark ringlets, and dark patent Mary Jane shoes. Despite her youthful look, she possesses significant age.

Her leadership includes morally contentious decisions, such as ordering the sacrifice of Kurusu Andō’s friend Yui to secure the Jewel of Faith—a utilitarian choice causing Kurusu’s desertion. She later demonstrates profound capability by facilitating Kureha Akabane’s revival after death, indicating access to powerful magic.

Her role frames the series’ narrative structure, with her signature mission-assignment phrase bookending the main storyline.