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Cleopatra appears across varied narratives. Historically, she is Cleopatra VII, the last pharaoh of Egypt's Ptolemaic Dynasty in the 1st century BC. Records counter seductress stereotypes, highlighting her intelligence, multilingualism, and economic skills that elevated Egypt to prosperity despite military vulnerabilities. Her political engagements with Rome involved relationships with Julius Caesar and Marcus Antonius, both loved by her and instrumental in Egypt's fate. She died by suicide after Egypt's fall to Rome.

In Fate/Grand Order, she manifests as an Assassin-class heroic spirit summoned by Ritsuka Fujimaru. Initially appearing in modern leather trousers and a jacket reflecting her preference for simplicity, she may reveal her historical attire as bonds strengthen. Her personality merges domineering authority with underlying kindness and attentiveness. She proclaims herself the absolute standard of beauty while dismissing others, yet shows genuine concern for their well-being. A perfectionist prone to excessive burdens, she seeks the Holy Grail to gain recognition for Caesar and their son Caesarion as legitimate heirs born of mutual love. She interacts respectfully with Egyptian pharaohs like Ozymandias and Nitocris, admires dynasty founder Iskandar, and harbors conflicted feelings toward Caesar's Throne of Heroes portrayal, criticizing his appearance while retaining affection.

In Majo Taisen - The War of Greedy Witches, she competes as the "Love Witch," driven by a desire for true mutual love after a lifetime of one-sided admiration. Ranked first in popularity due to supernatural beauty that charms all observers, she wields a "Magical Vestment" named *Aphrodite: Moira - Beauty to Court the Hand of Fate*, providing invulnerability to non-magical attacks and appearing as minimal gold armor with floating blue accessories. Her primary weapon is a khopesh sword. Her unique power, "Love Magic - A Charmed Hand," allows her to grant any wish by touching a sentient target or soul-imbued object with her right hand and issuing a command, enabling body control, deconstruction, heat manipulation, and energy projection. She employs this ruthlessly against those she deems unworthy of her love. During her battle with Himiko, she endures a broken arm and torso stab wound with extreme pain tolerance, and deduces opponents' abilities, such as recognizing Himiko's lie-based magic.

The 1970 film *Cleopatra* frames her story within a science-fiction context where future time travelers observe her era. Initially a politically marginalized figure with a plain appearance, she masters erotic arts for influence. Recruited by Egyptian rebels to assassinate Julius Caesar, she is magically transformed into stunning beauty by an ancient priestess. She captivates Caesar, bears his son Caesarion, and delays the assassination due to genuine affection. After Caesar's death, she forms a relationship with Marcus Antonius, whom she truly loves. When Octavian defeats Antony, she attempts to seduce Octavian but fails due to his homosexuality. Distraught by Antony's death and Octavian's rejection, she commits suicide via asp bite. The film incorporates surreal elements, psychedelic sequences, and deliberate anachronisms like Caesar using modern objects.