Misa Ono stands as one of the three central high school students featured in the Kyoto City Subway promotional media. She attends high school in Kyoto, relying on the subway for her daily commute. Her appearance features shoulder-length black hair styled in twintails, green eyes, glasses, and attire that includes thigh-high stockings.

Within her friend group, Misa embodies the cool, straight woman archetype, frequently serving as the grounded counterbalance to others. She is a guitar-playing enthusiast with dedicated interests in music and plant life. Her background establishes her as the childhood friend of Moe Uzumasa, another principal character.

The narrative follows Misa navigating daily life alongside Moe Uzumasa and Saki Matsuga as they utilize the subway system to explore Kyoto. Their experiences starting high school and embarking on commuting adventures across the city form the core of a 12-minute special episode. This animated episode, crowdfunded for the subway campaign, premiered on May 27, 2017.