Ptilol appears in the third season of *Sailor Moon Crystal* and the corresponding "Death Busters" arc of the manga as part of the Witches 5, serving the Death Busters organization under Professor Tomoe and Kaolinite. She exists intrinsically linked to Cyprine, together constituting the group's fifth member.
Ptilol manifests as a separate entity directly from Cyprine when Sailor Moon attacks Cyprine with the Moon Spiral Heart Attack. Official sources, including Naoko Takeuchi's Materials Collection, clarify Ptilol is not Cyprine's twin but an extension or manifestation of Cyprine's power, with both sharing a single existence despite appearing separate.
Physically, Ptilol possesses long red hair styled in a left-side braid, red eyes, and wears a black and red sleeveless dress with a black bow. Her attire includes red tights with black lace, short red heeled boots, small red earrings, black ribbons around her wrists, and a black star necklace. This design mirrors Cyprine's appearance, substituting a red color scheme for blue.
In battle, Ptilol and Cyprine operate as a coordinated unit. Their strategy involves one absorbing attacks with a magic staff while the other counterattacks, increasing their collective magical power. Within the manga continuity, they manipulate emotions, exploiting tensions among the Sailor Guardians to turn them against each other. Cyprine's signature "Ribbon Buster" attack releases painful energy ribbons, though Ptilol's use of this ability remains unconfirmed. Cyprine holds the highest rank among the Witches 5 at Level 999.
Ptilol's narrative concludes in both major continuities. In the manga and *Sailor Moon Crystal*, Super Sailor Moon destroys both Cyprine and Ptilol simultaneously using the Rainbow Moon Heartache attack. The classic 1990s anime sees them tricked by the Sailor Guardians into attacking each other, resulting in mutual destruction.
Ptilol never appears in a civilian disguise across any media, unlike other Witches 5 members, and remains the only Witch not featured in the *Sailor Moon* musicals. She makes a minor appearance in the video game *Sailor Moon: Another Story* as a boss encounter in Crystal Tokyo.