Ana Gram is a student at Root Academy and a Solver bearing the title "Da Vinci." Though male, Ana is often mistaken for a girl due to an effeminate appearance featuring long, straight brown hair and green eyes, accentuated by a preference for dresses and gowns. Ana typically wears a long white and yellow dress, an orange scarf, white bandages on the arms, and white slippers. Possessing a flamboyant yet socially awkward personality, Ana speaks in a distinctly feminine voice, frequently using third-person references like "Ana thinks." Distinctive speech includes phrases such as "Howawan,,, Fuwawaan..." and "The way Ana sees it." Ana openly enjoys cross-dressing and artistic endeavors like painting, often attributing creative inspiration to communications with the spirits of deceased artists.

Ana has an elder sister named Eve, who shares a connection with Mizerka of the Orpheus Order. A key childhood detail is that Ana never smiled until Eve painted a "picture of happiness." Beyond art, Ana demonstrates unexpected perceptiveness during puzzles, sometimes annoying opponents into underestimation. As a Solver, Ana collaborates with protagonists like Kaito Daimon, employing unique problem-solving methods that may involve improvisation or spiritual guidance. Ana notably won a school cross-dressing contest without formally entering. Primarily found in the art room and rarely attending other classes, Ana maintains friendships within the Root Academy group.

Ana's narrative presence diminishes in later seasons, particularly in season three, featuring only in a shared episode with Cubic Galois and without dedicated focus. Ana is not selected as a candidate for Orpheus possession. The name "Ana Gram" serves as a pun on "anagram."