Raised as Alphonse Kreutz, the male heir to Margrave Kreutz due to her family's political needs, Annelotte Kreutz developed traditionally masculine habits and traits while concealing her true gender. She served in the continent's strongest knightly order, the Knights of Margrave Kreutz, until Queen Claudette's forces destroyed her home. Wounded by the Queen's assassins, Annelotte fled to the Elven Forest, receiving swordsmanship training from Alleyne during her recovery. Distressed by the continent's descent into war under Claudette's tyranny, she founded the Rebellion Army, becoming its charismatic leader alongside fellow warriors sharing her fate. She formed close bonds with followers like Huit, Mirim, and Luna-Luna, affectionately calling them sisters-in-arms.

Annelotte possesses a strong sense of justice and knightly pride. Initially, her leadership was hampered by clumsiness and a tendency to shoulder burdens alone. She developed by learning to trust her companions' capabilities instead of protecting everyone single-handedly, solidifying her role as a unifying figure who inspired loyalty through determination and principle.

In combat, she wielded Grim Venus, a versatile weapon transforming between sword and spear. Using a horse-shaped crest accessory as a medium, she summoned Ambrosius, a demonic steed from the underworld. This connection stemmed from her half-demon heritage, shared with her long-lost sister Aldra. When emotionally distressed or pushed to physical limits, Annelotte underwent a demonic transformation known as the Possessed Knight state. This form granted heightened strength—enough to breach defenses like Mirim's Hyper Vibration Armor—but overwhelmed her rational mind, turning her into an indiscriminate berserker threatening allies and foes. Only interventions like Huit's embrace could subdue this state.

Her background included being the daughter of a human father and a demon mother ruling the Netherworld, initially unknown to her. Her biological sisterhood with Aldra was confirmed through later narrative developments. After overthrowing Queen Claudette and overseeing her homeland's reconstruction, Annelotte chose to wander the continent, training independently for future Queen's Blade tournaments while seeking personal growth.

Physically, Annelotte stood 159cm tall with lavender hair and blue eyes. Her attire featured ornate armor: a metal corset breastplate, arm guards with blue pauldrons, thigh-high metal boots, and a blue skirt. Her demonic form altered her appearance significantly, manifesting black hair, pointed ears, pale skin, red eyes, and golden armor.