Itsuko Hirai appears exclusively in the second episode of Lolita Anime, segment 2A titled "Itsuko Can Die Happy ~Altar of Sacrifice." Portrayed as an awkward schoolgirl, she harbors romantic feelings for an older male student, her senpai. During the narrative, a drunk salaryman attempts to assault Itsuko. Her senpai intervenes, halting the attack and offering immediate protection. Following this rescue, Itsuko seeks safety at her senpai's residence and remains there overnight.