Arnim Zola functions as a Hydra scientist specializing in genetic modification and manipulation. He directly creates and trains genetically altered teenagers—Makoto, Adi, Chloe, and Bruno—implanting distinct superhuman abilities like air manipulation, technology control, and shapeshifting. Throughout their upbringing, Zola and Hydra deceive these individuals, conditioning them to view the Avengers as villains while presenting themselves as mentors guiding them toward becoming superheroes.

Zola's scientific efforts are central to Hydra's Emerald Rain Project, an initiative possessing significant destructive potential. While the project's exact nature and full scope remain partially obscured, it is closely tied to the genetically modified teenagers under Zola's supervision. Makoto exhibits a direct connection to the project, manifesting unique emerald-hued powers during stress or crisis, including spontaneous generation of emerald energy wings. This suggests Zola's genetic experiments may have incorporated or activated latent capabilities linked to the project's objectives.

Consistent with other depictions, Zola utilizes a robotic body featuring his face as a holographic projection on the chest unit. This form provides enhanced durability and houses his consciousness. Zola employs technology enabling him to transfer his mind into other robotic bodies or computer systems if his current form is compromised, ensuring survival and allowing continued work for Hydra across locations and platforms.

Following the defection of Adi, Chloe, and Makoto to the Avengers, Zola persists in his genetic experiments and strategic operations for Hydra. He maintains contact with Bruno, who remains loyal after Red Skull reveals Hydra's true nature; Bruno's allegiance is partly secured by Hydra's promise to maintain his mechanical body's functionality. Zola continues supporting Hydra's broader goals, including the Emerald Rain Project, collaborating closely with Red Skull and assisting the Masters of Evil to further Hydra's agenda through alliances.

Operating under Red Skull's leadership within Hydra's hierarchy, Zola significantly contributes to Hydra's military and scientific endeavors. This includes outfitting Red Skull with advanced nanomachine armor designed to enhance his physical combat capabilities, demonstrating Zola's expertise in merging biological and technological systems. His work remains focused on achieving Hydra's aim of global dominance through superhuman agents, advanced weaponry, and large-scale destructive projects like the Emerald Rain initiative.