Mayuri Nakamura performs with the Alice in Theater troupe while assisting at her parents' coffee shop, leading to frequent tardiness for troupe activities. Her customer service background allows smooth interactions with fans and adept handling of audiences. She possesses long black hair extending to her arms, black eyes, and a distinctive outfit comprising a brown headband, gray dress with puffed sleeves, black skirt, white collar and cuffs, white apron, long white stockings, and black Mary Jane shoes.

Within the troupe, she maintains a sociable presence, often appearing in photographs alongside members like Seria Morino. During a guerilla performance at Radio Kaikan in Akihabara—staged to fund the financially struggling troupe—Mayuri participates when Airi Kagami initially declines. She witnesses pivotal events, including the activation of the Grand Material System during Alice in Theater’s Chrono Gazers performance, appearing visibly stunned alongside others as the world faces potential erasure.