Laura is a five-year-old female koala with tan/peach fur, black eyes featuring white highlights and two distinct eyelashes each, and a chocolate brown nose. Her typical attire includes a blue short-sleeved dress adorned with two small white buttons, paired with blue-and-white shoes showcasing yellow tongues/socks. For swimming, she wears a green one-piece suit topped with a plaid tutu skirt. Her sleepwear consists of a yellow nightgown featuring white frilly cuffs and orange slippers, while her underwear is a white tank top with frills and matching white briefs.

She is the daughter of Mommy and Papa Koala and the younger sister of Roobear, who functions as both her sibling and closest friend; family relationships anchor her interactions. Her social circle encompasses Floppy (a rabbit defined as her "non-boyfriend but best friend"), Mimi (a rabbit), Pamie (a penguin), Nick (a penguin), Betty (a koala), Diana the Moon Rabbit, Duckbill Platypus, Miss Lewis (her teacher), and Weather. Her connections with the Kangaroo Brothers (Colt, Walter, Horsey) and Mingle are more situational, marked by fluctuations between friendly and antagonistic dynamics; she regards the Kangaroo Brothers as frenemies, sometimes fearing them alongside the primary antagonist, the Wicked Wizard.

Her personality blends occasional brattiness with underlying kindness and caring tendencies. She displays strong will and nurturing behavior, exemplified in "Laura Finds an Egg," where she discovers and protects a turtle egg. After it hatches, she diligently cares for the baby turtle before releasing it into the wild, experiencing both sadness and pride in her caretaker role, highlighting her capacity for responsibility and emotional growth. Interactions also reveal occasional jealousy or rivalry, such as in "Who Will Be the Flower Queen?" where her competitive desire to win clashes with Roobear's loyalty to his girlfriend Betty, temporarily straining their sibling bond. Her role in group activities often involves curiosity and active participation in village adventures, including treasure hunts or environmental efforts like "Save the Eucalyptus."