Jesse serves as a primary antagonist within the narrative, operating as part of the Team Rocket trio alongside James and Meowth. Her core motivation remains capturing rare or powerful Pokémon to present to Giovanni for approval and reward, driving persistent ambition despite frequent failures. Within the specific events, she actively pursues the legendary Latias and Latios, recognizing their immense value. She demonstrates specific knowledge of Alto Mare's history and the Soul Dew's significance to these Eon Pokémon.

Teamwork with James and Meowth is a defining aspect, involving coordinated efforts to achieve their goals. Their plans typically involve elaborate, gadget-based schemes to capture targets. Jesse participates directly in creating a mechanical, animated Latios statue used as a lure for the real Latios and engages in the pursuit of both Latias and Latios across Alto Mare.

Failure is the consistent outcome of these efforts within this narrative, resulting in her and her teammates being ejected from the city following their defeat. Her actions exhibit no significant deviation from established personality traits or motivations, and no unique character development specific to this narrative is depicted beyond her participation in the central conflict involving the Eon Pokémon.