Mihoshi is a supporting character in Sasami: Magical Girls Club Season 2, where she serves as the homeroom teacher for Sasami Iwakura and Misao Shinohara at Tenko Academy. Her background in this series is that of a regular schoolteacher, though she is a counterpart to the Mihoshi character from the broader Tenchi Muyo! franchise, who in other continuities is often depicted as a clumsy but well-meaning Galaxy Police officer. In Sasami: Magical Girls Club, her role is entirely grounded in the everyday world; she is not a witch or magical girl herself, but she interacts with the main cast as an authority figure at school.

Personality-wise, Mihoshi is characterized by a combination of warmth and absentmindedness. She is genuinely caring and attentive toward her students, often offering encouragement and support. Despite her occasional clumsiness, she is consistently portrayed as professionally competent, capable of managing a classroom and fulfilling her teaching duties. Her motivations center on the well-being of her students, and she acts as a stable, reassuring presence in their daily lives.

Mihoshi's key relationships are primarily with her students, especially Sasami and Misao, whom she teaches in a standard homeroom capacity. She does not possess any magical abilities nor is she directly involved in the magical adventures or the conflicts of the second season, such as the Chief Sorceress’s schemes. Her role is that of an ordinary adult figure who provides a sense of normalcy and routine amidst the extraordinary events the girls experience. She does not undergo significant character development over the course of the season; her function remains consistent as a supportive, non-magical educator. Her notable traits include her patience, her gentle demeanor, and her ability to maintain order in the classroom despite her occasional scatterbrained moments. She has no supernatural abilities or special powers.