Misa Kuroi serves as vice president of Asaka Academy's Rifle Shooting Club. She possesses medium-length honey blonde hair, usually secured by a green headband, and violet eyes behind distinctive red rectangular glasses missing the upper frame. Her standard school uniform includes a long-sleeved dark navy blue jacket with parallel white lines on the sleeves, a gray pleated short skirt, long black socks, and burgundy laceless shoes.

Actively involved in club management, Misa carries responsibilities diligently. She approaches fellow club member Akira Shinonome to question why she wasn't informed of an upcoming practice match. While she can display a stern demeanor during interactions, she also expresses clear regret when feeling accountable for a first-year member's missed shot, revealing her conscientious nature.

Misa keenly observes rival schools' shooting club members, commenting on unexpected accomplishments during competitions. She openly admires Akira Shinonome's leadership qualities, explicitly stating Akira sounds like a genuine club president. Her actions include attempting to eavesdrop on conversations between club members like Erika Meinohama and Akira, indicating an interest in club dynamics. Her surname Kuroi (黒井) means "black well" or "black mine shaft," while her given name Misa (ミサ) is written in katakana, carrying no inherent meaning itself though subject to various kanji interpretations.