"Minami Kamakura High School Girls Cycling Club" centers around Hiromi Maiharu, a high school girl who moves to Kamakura and enrolls at Minami Kamakura High School. Having recently relocated from the countryside, Hiromi is unfamiliar with the area and initially struggles to navigate the city. Her life takes a turn when she encounters Tomoe Akitsuki, a classmate and avid cyclist, who introduces her to the world of cycling. Tomoe encourages Hiromi to join the school’s cycling club, which is in its early stages and lacks members.

The narrative explores Hiromi’s journey as she learns to ride a bicycle, gradually gaining confidence and skill. Alongside Tomoe, the club grows as other students join, including Shiki Kuroki, a quiet and reserved girl with a passion for cycling, and Fuyune Kamikura, a cheerful and energetic member who brings a lively dynamic to the group. Together, they embark on various cycling adventures, exploring the scenic landscapes of Kamakura and participating in local cycling events. The story emphasizes camaraderie, personal growth, and the joy of discovering new hobbies.

As the club members bond, they face challenges such as improving their cycling techniques, overcoming personal insecurities, and balancing their academic responsibilities with their passion for cycling. The series also highlights the beauty of Kamakura’s natural surroundings, with detailed depictions of its landmarks and seasonal changes, which serve as a backdrop for the characters’ experiences. Through their shared love for cycling, the girls develop lasting friendships and a deeper appreciation for their environment. The narrative maintains a lighthearted and slice-of-life tone, focusing on the everyday adventures and growth of the characters as they navigate high school life and their newfound passion for cycling.